Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease

Yvette Habraken, Patrick Sung, Louise Prakash, Satya Prakash

Resultado de la investigación: Articlerevisión exhaustiva

35 Citas (Scopus)


IN eukaryotes nucleotide excision repair of DNA damaged by ultraviolet radiation requires several gene products; defects in this process result in the cancer-prone syndrome xeroderma pigmento-sum (XP) in humans1,2. TheRAD2 gene is one of at least seven genes indispensable for excision repair in the yeast Saccharomyces cerevisiae2, and its encoded protein shares remarkable homology with the XP group-G gene product3. Here we overproduce the RAD2-encoded protein in S. cerevisiae, purify it to near homogeneity, and show that RAD2 protein in the presence of magnesium degrades circular single-stranded DNA. The RAD2 endonuclease is specific for single-stranded DNA as it does not act on doublestranded DNA. Given the absolute requirement for RAD2 in the incision step of excision repair, our findings directly implicate RAD2 protein and its human homologue XPG protein as a catalytic component that incises the damaged DNA strand during excision repair. Furthermore, our results indicate that eukaryotes probably employ two distinct endonuclease activities to mediate the dual incision at the damage site.

Idioma originalEnglish (US)
Páginas (desde-hasta)365-368
Número de páginas4
EstadoPublished - ene 1 1993
Publicado de forma externa

ASJC Scopus subject areas

  • General


Profundice en los temas de investigación de 'Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease'. En conjunto forman una huella única.

Citar esto