Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto; Anny Usheva; Gerard P. Zambetti; Jamil Momand; Nobuo Horikoshi; Roberto Weinmann; Arnold J. Levine; Thomas Shenk

Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto
, Anny Usheva
, Gerard P. Zambetti
, Jamil Momand
, Nobuo Horikoshi
, Roberto Weinmann
, Arnold J. Levine
, Thomas Shenk

Producción científica: Article › revisión exhaustiva

Resumen

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Idioma original	English (US)
Páginas (desde-hasta)	12028-12032
Número de páginas	5
Publicación	Proceedings of the National Academy of Sciences of the United States of America
Volumen	89
N.º	24
Estado	Published - 1992
Publicado de forma externa	Sí

ASJC Scopus subject areas

General

Otros archivos y enlaces

Citar esto

@article{477d7d602fce40949d3f2ff6100c9c99,

title = "Wild-type p53 binds to the TATA-binding protein and represses transcription",

abstract = "p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.",

author = "Edward Seto and Anny Usheva and Zambetti, \{Gerard P.\} and Jamil Momand and Nobuo Horikoshi and Roberto Weinmann and Levine, \{Arnold J.\} and Thomas Shenk",

year = "1992",

language = "English (US)",

volume = "89",

pages = "12028--12032",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "24",

}

TY - JOUR

T1 - Wild-type p53 binds to the TATA-binding protein and represses transcription

AU - Seto, Edward

AU - Usheva, Anny

AU - Zambetti, Gerard P.

AU - Momand, Jamil

AU - Horikoshi, Nobuo

AU - Weinmann, Roberto

AU - Levine, Arnold J.

AU - Shenk, Thomas

PY - 1992

Y1 - 1992

N2 - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

AB - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

UR - https://www.scopus.com/pages/publications/0027050648

UR - https://www.scopus.com/pages/publications/0027050648#tab=citedBy

M3 - Article

C2 - 1465435

AN - SCOPUS:0027050648

SN - 0027-8424

VL - 89

SP - 12028

EP - 12032

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

Wild-type p53 binds to the TATA-binding protein and represses transcription

Resumen

ASJC Scopus subject areas

Otros archivos y enlaces

Huella

Citar esto