Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto; Anny Usheva; Gerard P. Zambetti; Jamil Momand; Nobuo Horikoshi; Roberto Weinmann; Arnold J. Levine; Thomas Shenk

Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto, Anny Usheva, Gerard P. Zambetti, Jamil Momand, Nobuo Horikoshi, Roberto Weinmann, Arnold J. Levine, Thomas Shenk

Producción científica: Article › revisión exhaustiva

537 Citas (Scopus)

Resumen

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Idioma original	English (US)
Páginas (desde-hasta)	12028-12032
Número de páginas	5
Publicación	Proceedings of the National Academy of Sciences of the United States of America
Volumen	89
N.º	24
Estado	Published - 1992
Publicado de forma externa	Sí

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abstract = "p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.",

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year = "1992",

language = "English (US)",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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T1 - Wild-type p53 binds to the TATA-binding protein and represses transcription

AU - Seto, Edward

AU - Usheva, Anny

AU - Zambetti, Gerard P.

AU - Momand, Jamil

AU - Horikoshi, Nobuo

AU - Weinmann, Roberto

AU - Levine, Arnold J.

AU - Shenk, Thomas

PY - 1992

Y1 - 1992

N2 - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

AB - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

Wild-type p53 binds to the TATA-binding protein and represses transcription

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