Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: Results from single lumbar puncture sampling technique

Francisco A. Moreno, Cameron McGavin, T. Phillip Malan, Alan J. Gelenberg, George R. Heninger, Aleksander A. Mathé, Pedro L. Delgado

Resultado de la investigación: Articlerevisión exhaustiva

41 Citas (Scopus)

Resumen

Plasma tryptophan (Trp) depletion is a commonly used tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders. This study measured the cerebrospinal fluid (CSF) monoamine metabolite response to Trp depletion and control testing in five healthy subjects utilizing a single lumbar puncture. Testing was done in a placebo-controlled, double-blind, randomized, cross-over design. Plasma-free and total Trp levels and behavioural ratings were obtained prior to and 5 h after ingestion of each amino-acid drink. CSF was obtained by performing a standard lumbar puncture 7 h after ingestion of the drink. Compared to control testing, Trp depletion caused a significant decrease of CSF 5-hydroxyindoleacetic acid (5-HIAA) (p = 0.03), but not of homovanillic acid or 3-methoxy-4-hydroxy-phenylglycol. Behavioural ratings were minimally affected in all subjects. This confirms that plasma Trp depletion reduces central nervous system measures of 5-HT function and suggests that the single lumbar puncture technique may be sufficient to detect the extent of CSF 5-HIAA changes during Trp depletion studies.

Idioma originalEnglish (US)
Páginas (desde-hasta)277-283
Número de páginas7
PublicaciónInternational Journal of Neuropsychopharmacology
Volumen3
N.º4
DOI
EstadoPublished - 2000
Publicado de forma externa

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health
  • Pharmacology (medical)

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