Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway

Bruno Doiron, Marie Hélène Cuif, Ruihuan Chen, Axel Kahn

Producción científica: Articlerevisión exhaustiva

119 Citas (Scopus)

Resumen

Glucose catabolism induces the expression of the L-type pyruvate kinase (L-PK) gene through the glucose response element (GIRE). The metabolic pathway used by glucose after its phosphorylation to glucose 6-phosphate by glucokinase to induce L-PK gene expression in hepatocytes remains unknown. The sugar alcohol xylitol is metabolized to xylulose 5-phosphate, an intermediate of the nonoxidative branch of the pentose phosphate pathway. In this study, we demonstrated that xylitol at low concentration (0.5 mM) induced the expression of the L-PK/CAT construct in glucose-responsive mhAT3F hepatoma cells at the same level as 20 mM glucose, while it did not affect intracellular concentration of glucose 6-phosphate significantly. The effect of xylitol on the induction of the L-PK gene expression was noncumulative with that of glucose since 20 mM glucose plus 5 mM xylitol induced the expression of the L-PK/CAT construct similarly to 20 mM glucose alone. In hepatocytes in primary culture, 5 mM xylitol induced accumulation of the L- PK mRNA even in the absence of insulin. Furthermore, the response to xylitol as well as glucose required the presence of a functional GIRE. It can be assumed from these results that glucose induces the expression of the L-PK gene through the nonoxidative branch of the pentose phosphate pathway. The effect of xylitol at low concentration suggests that the glucose signal to the transcriptional machinery is mediated by xylulose 5-phosphate.

Idioma originalEnglish (US)
Páginas (desde-hasta)5321-5324
Número de páginas4
PublicaciónJournal of Biological Chemistry
Volumen271
N.º10
DOI
EstadoPublished - mar 8 1996
Publicado de forma externa

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Huella

Profundice en los temas de investigación de 'Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway'. En conjunto forman una huella única.

Citar esto