Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex

Tabish Hussain; Dhurjhoti Saha; Gunjan Purohit; Anirban Kar; Anand Kishore Mukherjee; Shalu Sharma; Suman Sengupta; Parashar Dhapola; Basudeb Maji; Sreekanth Vedagopuram; Nobuko T. Horikoshi; Nobuo Horikoshi; Raj K. Pandita; Santanu Bhattacharya; Avinash Bajaj; Jean François Riou; Tej K. Pandita; Shantanu Chowdhury

doi:10.1038/s41598-017-11177-1

Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex

Tabish Hussain, Dhurjhoti Saha, Gunjan Purohit, Anirban Kar, Anand Kishore Mukherjee, Shalu Sharma, Suman Sengupta, Parashar Dhapola, Basudeb Maji, Sreekanth Vedagopuram, Nobuko T. Horikoshi, Nobuo Horikoshi, Raj K. Pandita, Santanu Bhattacharya, Avinash Bajaj, Jean François Riou, Tej K. Pandita, Shantanu Chowdhury

Producción científica: Article › revisión exhaustiva

44 Citas (Scopus)

Resumen

We observed extra-telomeric binding of the telomere repeat binding factor TRF2 within the promoter of the cyclin-dependent kinase CDKNIA (p21/CIP1/WAF1). This result in TRF2 induced transcription repression of p21. Interestingly, p21 repression was through engagement of the REST-coREST-LSD1-repressor complex and altered histone marks at the p21 promoter in a TRF2-dependent fashion. Furthermore, mutational analysis shows p21 repression requires interaction of TRF2 with a p21 promoter G-quadruplex. Physiologically, TRF2-mediated p21 repression attenuated drug-induced activation of cellular DNA damage response by evading G2/M arrest in cancer cells. Together these reveal for the first time role of TRF2 in REST-repressor complex mediated transcription repression.

Idioma original	English (US)
Número de artículo	11541
Publicación	Scientific reports
Volumen	7
N.º	1
DOI	https://doi.org/10.1038/s41598-017-11177-1
Estado	Published - dic 1 2017
Publicado de forma externa	Sí

ASJC Scopus subject areas

General

Acceder al documento

10.1038/s41598-017-11177-1

Otros archivos y enlaces

Citar esto

Hussain, T., Saha, D., Purohit, G., Kar, A., Kishore Mukherjee, A., Sharma, S., Sengupta, S., Dhapola, P., Maji, B., Vedagopuram, S., Horikoshi, N. T., Horikoshi, N., Pandita, R. K., Bhattacharya, S., Bajaj, A., Riou, J. F., Pandita, T. K., & Chowdhury, S. (2017). Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex. Scientific reports, 7(1), Artículo 11541. https://doi.org/10.1038/s41598-017-11177-1

Hussain, T, Saha, D, Purohit, G, Kar, A, Kishore Mukherjee, A, Sharma, S, Sengupta, S, Dhapola, P, Maji, B, Vedagopuram, S, Horikoshi, NT, Horikoshi, N, Pandita, RK, Bhattacharya, S, Bajaj, A, Riou, JF, Pandita, TK & Chowdhury, S 2017, 'Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex', Scientific reports, vol. 7, n.º 1, 11541. https://doi.org/10.1038/s41598-017-11177-1

@article{e6c7d81c9e474b49b0b24f24269f06f6,

title = "Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex",

abstract = "We observed extra-telomeric binding of the telomere repeat binding factor TRF2 within the promoter of the cyclin-dependent kinase CDKNIA (p21/CIP1/WAF1). This result in TRF2 induced transcription repression of p21. Interestingly, p21 repression was through engagement of the REST-coREST-LSD1-repressor complex and altered histone marks at the p21 promoter in a TRF2-dependent fashion. Furthermore, mutational analysis shows p21 repression requires interaction of TRF2 with a p21 promoter G-quadruplex. Physiologically, TRF2-mediated p21 repression attenuated drug-induced activation of cellular DNA damage response by evading G2/M arrest in cancer cells. Together these reveal for the first time role of TRF2 in REST-repressor complex mediated transcription repression.",

author = "Tabish Hussain and Dhurjhoti Saha and Gunjan Purohit and Anirban Kar and {Kishore Mukherjee}, Anand and Shalu Sharma and Suman Sengupta and Parashar Dhapola and Basudeb Maji and Sreekanth Vedagopuram and Horikoshi, {Nobuko T.} and Nobuo Horikoshi and Pandita, {Raj K.} and Santanu Bhattacharya and Avinash Bajaj and Riou, {Jean Fran{\c c}ois} and Pandita, {Tej K.} and Shantanu Chowdhury",

note = "Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = dec,

day = "1",

doi = "10.1038/s41598-017-11177-1",

language = "English (US)",

volume = "7",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Research",

number = "1",

}

TY - JOUR

T1 - Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex

AU - Hussain, Tabish

AU - Saha, Dhurjhoti

AU - Purohit, Gunjan

AU - Kar, Anirban

AU - Kishore Mukherjee, Anand

AU - Sharma, Shalu

AU - Sengupta, Suman

AU - Dhapola, Parashar

AU - Maji, Basudeb

AU - Vedagopuram, Sreekanth

AU - Horikoshi, Nobuko T.

AU - Horikoshi, Nobuo

AU - Pandita, Raj K.

AU - Bhattacharya, Santanu

AU - Bajaj, Avinash

AU - Riou, Jean François

AU - Pandita, Tej K.

AU - Chowdhury, Shantanu

PY - 2017/12/1

Y1 - 2017/12/1

N2 - We observed extra-telomeric binding of the telomere repeat binding factor TRF2 within the promoter of the cyclin-dependent kinase CDKNIA (p21/CIP1/WAF1). This result in TRF2 induced transcription repression of p21. Interestingly, p21 repression was through engagement of the REST-coREST-LSD1-repressor complex and altered histone marks at the p21 promoter in a TRF2-dependent fashion. Furthermore, mutational analysis shows p21 repression requires interaction of TRF2 with a p21 promoter G-quadruplex. Physiologically, TRF2-mediated p21 repression attenuated drug-induced activation of cellular DNA damage response by evading G2/M arrest in cancer cells. Together these reveal for the first time role of TRF2 in REST-repressor complex mediated transcription repression.

AB - We observed extra-telomeric binding of the telomere repeat binding factor TRF2 within the promoter of the cyclin-dependent kinase CDKNIA (p21/CIP1/WAF1). This result in TRF2 induced transcription repression of p21. Interestingly, p21 repression was through engagement of the REST-coREST-LSD1-repressor complex and altered histone marks at the p21 promoter in a TRF2-dependent fashion. Furthermore, mutational analysis shows p21 repression requires interaction of TRF2 with a p21 promoter G-quadruplex. Physiologically, TRF2-mediated p21 repression attenuated drug-induced activation of cellular DNA damage response by evading G2/M arrest in cancer cells. Together these reveal for the first time role of TRF2 in REST-repressor complex mediated transcription repression.

UR - http://www.scopus.com/inward/record.url?scp=85029488517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029488517&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-11177-1

DO - 10.1038/s41598-017-11177-1

M3 - Article

C2 - 28912501

AN - SCOPUS:85029488517

SN - 2045-2322

VL - 7

JO - Scientific reports

JF - Scientific reports

IS - 1

M1 - 11541

ER -

Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex

Resumen

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto