TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

Jyotika Sharma; Bibhuti B. Mishra; Qun Li; Judy M. Teale

doi:10.1016/j.cellimm.2011.03.023

TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

Jyotika Sharma, Bibhuti B. Mishra, Qun Li, Judy M. Teale

Department of Developmental Dentistry

Producción científica: Article › revisión exhaustiva

15 Citas (Scopus)

Resumen

The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.

Idioma original	English (US)
Páginas (desde-hasta)	69-73
Número de páginas	5
Publicación	Cellular Immunology
Volumen	269
N.º	2
DOI	https://doi.org/10.1016/j.cellimm.2011.03.023
Estado	Published - 2011

ASJC Scopus subject areas

Immunology

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10.1016/j.cellimm.2011.03.023

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title = "TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu",

abstract = "The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.",

keywords = "Elongation factor-Tu, Francisella, Inflammation, Innate immune response, Toll-like receptor, Tularemia",

author = "Jyotika Sharma and Mishra, {Bibhuti B.} and Qun Li and Teale, {Judy M.}",

note = "Funding Information: The authors thank Drs. Collin Manoil and Larry Gallagher, University of Washington for kindly providing the F. novicida mutant strain; and Dr. Derek P. Thomas, University of Texas at San Antonio for technical help with 2DE analysis. This work was supported by National Institute of Health Grants 1P01A10157986 , NS35974 and AI 59703 to JMT and American Heart Association Grant 10BGIA4300041 to JS.",

year = "2011",

doi = "10.1016/j.cellimm.2011.03.023",

language = "English (US)",

volume = "269",

pages = "69--73",

journal = "Cellular Immunology",

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T1 - TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

AU - Sharma, Jyotika

AU - Mishra, Bibhuti B.

AU - Li, Qun

AU - Teale, Judy M.

N1 - Funding Information: The authors thank Drs. Collin Manoil and Larry Gallagher, University of Washington for kindly providing the F. novicida mutant strain; and Dr. Derek P. Thomas, University of Texas at San Antonio for technical help with 2DE analysis. This work was supported by National Institute of Health Grants 1P01A10157986 , NS35974 and AI 59703 to JMT and American Heart Association Grant 10BGIA4300041 to JS.

PY - 2011

Y1 - 2011

N2 - The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.

AB - The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.

KW - Elongation factor-Tu

KW - Francisella

KW - Inflammation

KW - Innate immune response

KW - Toll-like receptor

KW - Tularemia

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DO - 10.1016/j.cellimm.2011.03.023

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AN - SCOPUS:79957665364

SN - 0008-8749

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SP - 69

EP - 73

JO - Cellular Immunology

JF - Cellular Immunology

IS - 2

ER -

TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

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