Tissue inhibitor of metalloproteinase-2 inhibits burn-induced derangements and hyperpermeability in microvascular endothelial cells Presented as poster presentation at the American Pediatric Surgical Association 45th Annual Meeting, May 29 to June 1, 2014, Phoenix, Arizona.

Katie Wiggins-Dohlvik, Ryan P. Oakley, Min Suk Han, Hayden W. Stagg, Himakarnika Alluri, Chinchusha A. Shaji, Matthew L. Davis, Binu Tharakan

Producción científica: Articlerevisión exhaustiva

10 Citas (Scopus)

Resumen

Background Burns induce microvascular hyperpermeability. We hypothesize that this occurs partly through an imbalance between matrix metalloproteinases (MMPs) and endogenous MMP inhibitors such as tissue inhibitors of metalloproteinases (TIMPs), and that such derangements can be attenuated with the use of TIMP-2. Method Rats underwent either sham or burn: serum and tissue were collected. Western blot was used to examine MMP-9 and TIMP-2 levels and MMP activity was assayed from lung tissue. Rat lung microvascular endothelial cells were used to assess monolayer permeability and evaluate the adherens junction proteins β-catenin, vascular endothelial cadherin and filamentous actin after exposure to burn serum ± TIMP-2. Results Lung tissue from burn animals showed increased MMP activity, decreased levels of TIMP-2, and no difference in levels of active MMP-9 in burn vs control groups. Burn serum increased monolayer permeability, damaged adherens junction proteins, and incited actin stress fiber formation; TIMP-2 attenuated these derangements. Conclusions Burns may lower TIMP-2 levels and increase MMP activity and that TIMP-2 application in vitro may attenuate burn-induced hyperpermeability and decreases damage to endothelial structural proteins. These links warrant further investigation.

Idioma originalEnglish (US)
Páginas (desde-hasta)197-205
Número de páginas9
PublicaciónAmerican journal of surgery
Volumen211
N.º1
DOI
EstadoPublished - ene 1 2016
Publicado de forma externa

ASJC Scopus subject areas

  • Surgery

Huella

Profundice en los temas de investigación de 'Tissue inhibitor of metalloproteinase-2 inhibits burn-induced derangements and hyperpermeability in microvascular endothelial cells Presented as poster presentation at the American Pediatric Surgical Association 45th Annual Meeting, May 29 to June 1, 2014, Phoenix, Arizona.'. En conjunto forman una huella única.

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