The use of polyacrylamide gels that can be dissassembled and reassembled to permit a wide variety of electrophoretic procedures with a common polymerization history

A demountable polyacrylamide gel system is described that uses commercial components and can be adapted to any slab system. The use of GeldBond PAG film produces polyacrylamide gels that can be easily handled and allows separation of the conditions needed for polymerization from those for electrophoresis. Polymerization is performed with one common protocol, and the gel is disassembled. Gels can then be soaked or stored in any desired solutions, followed by reassembly of the equilibrated gel and electrophoresis. This procedure allows for a wide range of electrophoretic conditions starting with a single type of polymerized gel as well as permitting use of systems that have technical difficulties under normal cirumstances such as horizontal gels or those containing the cationic detergent, cetyltrimethylammonium bromide.