The use of flurescent dyes to measure membrane potentials: A response

Thomas C. Smith

Resultado de la investigación: Articlerevisión exhaustiva

22 Citas (Scopus)

Resumen

The use of fluorescent cyanine dyes to estimate membrane potential in cell suspensions has been considered. Several problems related to the application of the dyes have been reviewed. These problems include: (1) alteration of the membrane potential (Em) and factors involved in establishing Em by the dyes themselves, (2) the effect of altered energy metabolism on the fluorescent response of the dyes and on Em, and (3) calibration of dye fluorescence. Recent reports that advocate the use of the fluorescent dyes are misleading.

Idioma originalEnglish (US)
Páginas (desde-hasta)302-305
Número de páginas4
PublicaciónJournal of Cellular Physiology
Volumen112
N.º2
DOI
EstadoPublished - ago. 1982
Publicado de forma externa

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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