The use of Chinese hamster ovary (CHO) cells in the study of ion channels

Nikita Gamper; James D. Stockand; Mark S. Shapiro

doi:10.1016/j.vascn.2004.08.008

The use of Chinese hamster ovary (CHO) cells in the study of ion channels

Nikita Gamper, James D. Stockand, Mark S. Shapiro

Department of Cellular & Integrative Physiology

Producción científica: Article › revisión exhaustiva

95 Citas (Scopus)

Resumen

The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science. This paper discusses the different features of CHO cell physiology as well as the specific aspects of using these cells for ion channel studies; among the discussed features are the culturing and transfection of CHO cells, details of electrophysiological recordings from them and applications for the study of ion channel physiology and pharmacology. Examples of successful reconstitution of mammalian ion channels in CHO cells discussed in the paper include reconstitution of KCNQ channel regulation by muscarinic acetylcholine receptors and the study of the amiloride-sensitivity of epithelial sodium channels (ENaC).

Idioma original	English (US)
Páginas (desde-hasta)	177-185
Número de páginas	9
Publicación	Journal of Pharmacological and Toxicological Methods
Volumen	51
N.º	3 SPEC. ISS.
DOI	https://doi.org/10.1016/j.vascn.2004.08.008
Estado	Published - 2005

ASJC Scopus subject areas

Toxicology
Pharmacology

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title = "The use of Chinese hamster ovary (CHO) cells in the study of ion channels",

abstract = "The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science. This paper discusses the different features of CHO cell physiology as well as the specific aspects of using these cells for ion channel studies; among the discussed features are the culturing and transfection of CHO cells, details of electrophysiological recordings from them and applications for the study of ion channel physiology and pharmacology. Examples of successful reconstitution of mammalian ion channels in CHO cells discussed in the paper include reconstitution of KCNQ channel regulation by muscarinic acetylcholine receptors and the study of the amiloride-sensitivity of epithelial sodium channels (ENaC).",

keywords = "CHO cells, ENaC, KCNQ, Methods, Muscarinic receptors, Patch clamp, Potassium channels, Transfection",

author = "Nikita Gamper and Stockand, {James D.} and Shapiro, {Mark S.}",

note = "Funding Information: This work was supported by the National Institutes of Health grants R01 NS43394 to MSS and RO1 DK59594 to JDS, and by an American Heart Association-Texas postdoctoral fellowship grant 0325120Y to NG.",

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T1 - The use of Chinese hamster ovary (CHO) cells in the study of ion channels

AU - Gamper, Nikita

AU - Stockand, James D.

AU - Shapiro, Mark S.

N1 - Funding Information: This work was supported by the National Institutes of Health grants R01 NS43394 to MSS and RO1 DK59594 to JDS, and by an American Heart Association-Texas postdoctoral fellowship grant 0325120Y to NG.

PY - 2005

Y1 - 2005

N2 - The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science. This paper discusses the different features of CHO cell physiology as well as the specific aspects of using these cells for ion channel studies; among the discussed features are the culturing and transfection of CHO cells, details of electrophysiological recordings from them and applications for the study of ion channel physiology and pharmacology. Examples of successful reconstitution of mammalian ion channels in CHO cells discussed in the paper include reconstitution of KCNQ channel regulation by muscarinic acetylcholine receptors and the study of the amiloride-sensitivity of epithelial sodium channels (ENaC).

AB - The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science. This paper discusses the different features of CHO cell physiology as well as the specific aspects of using these cells for ion channel studies; among the discussed features are the culturing and transfection of CHO cells, details of electrophysiological recordings from them and applications for the study of ion channel physiology and pharmacology. Examples of successful reconstitution of mammalian ion channels in CHO cells discussed in the paper include reconstitution of KCNQ channel regulation by muscarinic acetylcholine receptors and the study of the amiloride-sensitivity of epithelial sodium channels (ENaC).

KW - CHO cells

KW - ENaC

KW - KCNQ

KW - Methods

KW - Muscarinic receptors

KW - Patch clamp

KW - Potassium channels

KW - Transfection

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The use of Chinese hamster ovary (CHO) cells in the study of ion channels

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