TY - JOUR
T1 - The type of chemical fixative affects the immunohistochemical localization of transforming growth factor α in rat colon crypts
AU - Hardman, W. Elaine
AU - Cameron, Ivan L.
N1 - Funding Information:
Our thanks to Joshua Johnson for technical assistance with this research. This work was supported by Veterans Administration Merit Award 93-001, NCI Cancer Center Support Grant P30 CA 54174 and the Cancer Research Foundation of America
PY - 1998
Y1 - 1998
N2 - The effects of the different fixation agents, Omnifix, 10% neutral- buffered formalin, 70% ethanol, acetone, 10% neutral-buffered formalin, for 2h followed by 70% ethanol for 10 h or 40% paraformaldehyde on the immunohistochemical localization of transforming growth factor α in rat colon crypts were studied. Segments of rat descending colon were fixed and then processed for immunohistochemistry using a monoclonal antibody to the growth factor. The results showed that the pattern of transforming growth factor α staining in the colonic crypts differed and was distinct for each fixative.
AB - The effects of the different fixation agents, Omnifix, 10% neutral- buffered formalin, 70% ethanol, acetone, 10% neutral-buffered formalin, for 2h followed by 70% ethanol for 10 h or 40% paraformaldehyde on the immunohistochemical localization of transforming growth factor α in rat colon crypts were studied. Segments of rat descending colon were fixed and then processed for immunohistochemistry using a monoclonal antibody to the growth factor. The results showed that the pattern of transforming growth factor α staining in the colonic crypts differed and was distinct for each fixative.
UR - http://www.scopus.com/inward/record.url?scp=0031901586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031901586&partnerID=8YFLogxK
U2 - 10.1023/A:1003272109137
DO - 10.1023/A:1003272109137
M3 - Article
AN - SCOPUS:0031901586
SN - 1567-2379
VL - 30
SP - 301
EP - 305
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
IS - 5
ER -