Resumen
Biglycan (bgn) is a small leucine-rich proteoglycan enriched in extracellular matrices of skeletal tissues. Bgn-deficient mice develop age-related osteopenia with a phenotype that resembles osteoporosis and premature arthritis. In the present study, we have examined the differentiation of bgn-deficient osteoblasts from neonatal murine calvariae and found that the absence of bgn caused less BMP-4 binding, which reduced the sensitivity of osteoblasts to BMP-4 stimulation. The loss of sensitivity resulted in a reduction of Cbfa1 expression, which ultimately led to a defect in the differentiation of osteoblasts. However, the response of bgn-deficient osteoblasts to BMP-4 was completely rescued by reintroduction of biglycan by viral transfection. We propose that biglycan modulates BMP-4-induced signaling to control osteoblast differentiation.
Idioma original | English (US) |
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Páginas (desde-hasta) | 948-958 |
Número de páginas | 11 |
Publicación | FASEB Journal |
Volumen | 18 |
N.º | 9 |
DOI | |
Estado | Published - jun 2004 |
Publicado de forma externa | Sí |
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Biochemistry
- Biotechnology