TY - JOUR
T1 - The response of human colonocytes to folate deficiency in vitro
T2 - Functional and proteomic analyses
AU - Duthie, Susan J.
AU - Mavrommatis, Yiannis
AU - Rucklidge, Gary
AU - Reid, Martin
AU - Duncan, Gary
AU - Moyer, Mary P.
AU - Pirie, Lynn P.
AU - Bestwick, Charles S.
PY - 2008/8
Y1 - 2008/8
N2 - Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].
AB - Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].
KW - DNA repair, apoptosis, proteomics
KW - Folic acid deficiency
KW - Genomic stability
KW - Human colonocytes
KW - NCM460
UR - http://www.scopus.com/inward/record.url?scp=53049085981&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=53049085981&partnerID=8YFLogxK
U2 - 10.1021/pr700751y
DO - 10.1021/pr700751y
M3 - Article
C2 - 18597513
AN - SCOPUS:53049085981
SN - 1535-3893
VL - 7
SP - 3254
EP - 3266
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 8
ER -