The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis

Michael W. Lehker, Rossana Arroyo, John F. Alderete

Resultado de la investigación: Articlerevisión exhaustiva

99 Citas (Scopus)

Resumen

Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically mediated by iron; parasites cultured in a low-iron medium in the presence of salts other than iron were unresponsive to changes in adherence levels. Expression ofthe higher adherence property, by parasites grown first in low-iron medium followed by supplementation with iron, was a function of time, and the extent of cytoadherence was proportional to the concentration of iron added to the medium. Lactoferrin, an important iron source for trichomonads at the site of infection, elevated adherence of the parasite to epithelial cells, demonstrating the likely in vivo modulation of adherence by iron. The alteration of levels of adherence caused by iron was determined to be a reflection of gene expression of previously characterized trichomonad adhesins. Parasites grown under iron-replete conditions had higher quantities of surface-exposed adhesins, and this was a result ofincreased synthesis ofadhesins. Actinomycin D and a-amanitin prevented expression of adhesin molecules, which resulted in decreased cytoadherence, showing that adhesin synthesis was dependent on gene transcription. Data indicated that genes encoding the four trichomonad adhesins are coordinately regulated by iron.

Idioma originalEnglish (US)
Páginas (desde-hasta)311-318
Número de páginas8
PublicaciónJournal of Experimental Medicine
Volumen174
N.º2
DOI
EstadoPublished - ago. 1 1991
Publicado de forma externa

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Huella

Profundice en los temas de investigación de 'The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis'. En conjunto forman una huella única.

Citar esto