The Post-Synaptic Function of Brca2

Charles X. Wang, Judit Jimenez-Sainz, Ryan B. Jensen, Alexander V. Mazin

Resultado de la investigación: Articlerevisión exhaustiva

3 Citas (Scopus)

Resumen

Homologous Recombination (HR) is a high-fidelity process with a range of biologic functions from generation of genetic diversity to repair of DNA double-strand breaks (DSBs). In mammalian cells, BRCA2 facilitates the polymerization of RAD51 onto ssDNA to form a presynaptic nucleoprotein filament. This filament can then strand invade a homologous dsDNA to form the displacement loop (D-loop) structure leading to the eventual DSB repair. Here, we have found that RAD51 in stoichiometric excess over ssDNA can cause D-loop disassembly in vitro; furthermore, we show that this RAD51 activity is countered by BRCA2. These results demonstrate that BRCA2 may have a previously unexpected activity: regulation of HR at a post-synaptic stage by modulating RAD51-mediated D-loop dissociation. Our in vitro results suggest a mechanistic underpinning of homeostasis between RAD51 and BRCA2, which is an important factor of HR in mammalian cells.

Idioma originalEnglish (US)
Número de artículo4554
PublicaciónScientific reports
Volumen9
N.º1
DOI
EstadoPublished - dic. 1 2019
Publicado de forma externa

ASJC Scopus subject areas

  • General

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