The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture

Delio P. Ortegon, Michael R. Davis, Patricia S. Dixon, David L. Smith, John D. Josephs, Deborah L. Mueller, Donald H. Jenkins, Jeffrey D. Kerby

Producción científica: Articlerevisión exhaustiva

19 Citas (Scopus)

Resumen

Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb.

Idioma originalEnglish (US)
Páginas (desde-hasta)1068-1072
Número de páginas5
PublicaciónJournal of Trauma - Injury, Infection and Critical Care
Volumen53
N.º6
DOI
EstadoPublished - dic 1 2002
Publicado de forma externa

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Surgery

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