The DNA cytosine deaminase APOBEC3H haplotype i likely contributes to breast and lung cancer mutagenesis

Gabriel J. Starrett, Elizabeth M. Luengas, Jennifer L. McCann, Diako Ebrahimi, Nuri A. Temiz, Robin P. Love, Yuqing Feng, Madison B. Adolph, Linda Chelico, Emily K. Law, Michael A. Carpenter, Reuben S. Harris

Producción científica: Articlerevisión exhaustiva

119 Citas (Scopus)

Resumen

Cytosine mutations within TCA/T motifs are common in cancer. A likely cause is the DNA cytosine deaminase APOBEC3B (A3B). However, A3B-null breast tumours still have this mutational bias. Here we show that APOBEC3H haplotype I (A3H-I) provides a likely solution to this paradox. A3B-null tumours with this mutational bias have at least one copy of A3H-I despite little genetic linkage between these genes. Although deemed inactive previously, A3H-I has robust activity in biochemical and cellular assays, similar to A3H-II after compensation for lower protein expression levels. Gly105 in A3H-I (versus Arg105 in A3H-II) results in lower protein expression levels and increased nuclear localization, providing a mechanism for accessing genomic DNA. A3H-I also associates with clonal TCA/T-biased mutations in lung adenocarcinoma suggesting this enzyme makes broader contributions to cancer mutagenesis. These studies combine to suggest that A3B and A3H-I, together, explain the bulk of € APOBEC signature' mutations in cancer.

Idioma originalEnglish (US)
Número de artículo12918
PublicaciónNature communications
Volumen7
DOI
EstadoPublished - sept 21 2016
Publicado de forma externa

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General Physics and Astronomy

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