The chemokine CCL5 is essential for leukocyte recruitment in a model of severe Herpes simplex encephalitis

Márcia Carvalho Vilela, Daniel Santos Mansur, Norinne Lacerda-Queiroz, David Henrique Rodrigues, Graciela Kunrath Lima, Rosa Maria Esteves Arantes, Erna Geessien Kroon, Marco Antônio Da Silva Campos, Mauro Martins Teixeira, Antônio Lúcio Teixeira

Producción científica: Conference contribution

42 Citas (Scopus)


The Herpes simplex virus-1 (HSV-1) is responsible for several clinical manifestations in humans, including encephalitis. To induce encephalitis, C57BL/6 mice were inoculated with 104 plaque-forming cells of HSV-1 by the intracranial route. Met-RANTES (regulated upon activation, normal T cell expressed and presumably secreted) (10 μg/mouse), a CC chemokine family receptor (CCR)1 and CCR5 antagonist, was given subcutaneously the day before, immediately after, and at days 1, 2, and 3 after infection. Treatment with Met-RANTES had no effect on the viral titers. In contrast, intravital microscopy revealed that treatment with Met-RANTES decreased the number of leukocytes adherent to the pial microvasculature at days 1 and 3 after infection. The levels of the chemokines CCL3, CCL5, CXCL1, and CXCL9 increased after infection and were enhanced further by the treatment with Met-RANTES. Treatment with a polyclonal anti-CCL5 antibody 2 h before the intravital microscopy decreased leukocyte adhesion in the microcirculation of infected mice. In conclusion, CCL5, a chemokine that binds to CCR1 and CCR5, is essential for leukocyte adhesion during HSV-1 encephalitis. However, blocking of CCR1 and CCR5 did not affect HSV-1 replication, suggesting that other immune mechanisms are involved in the process of infection control.

Idioma originalEnglish (US)
Título de la publicación alojadaNeuroimmunomodulation
Subtítulo de la publicación alojadaFrom Fundamental Biology to Therapy
EditorialBlackwell Publishing Inc.
Número de páginas8
ISBN (versión impresa)9781573317467
EstadoPublished - feb 2009
Publicado de forma externa

Serie de la publicación

NombreAnnals of the New York Academy of Sciences
ISSN (versión impresa)0077-8923
ISSN (versión digital)1749-6632

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • History and Philosophy of Science


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