TY - JOUR
T1 - The binding of a polyclonal antibody against human band 3to in vitro aged erythrocytes
AU - Klinger, Matthias H.F.
AU - Halbhuber, Karl Jurgen
AU - Linss, Werner
AU - Feuerstein, Herwart
AU - Gaczynska, Maria
PY - 1990
Y1 - 1990
N2 - In vitro aged human erythrocytes were checked for their anti-band-3-antibody loading by means of a protein A-goldtechnique. The in vitro-ageing procedures are introduced as an alternative to the questionable preparation of in vivo aged cells. They include the influences of NaF in various concentrations, of heat (49 °C) and urea. After the treatment with 20 mmol/1 NaF and more, the IgG loading increases significantly. In contrast to that, however, exovesiculation induced by heat and urea lower the IgG loading. Possible mechanisms for the growing accessibility of the epitopes concerned are discussed in connection with changes occurring during the physiological ageing processes.
AB - In vitro aged human erythrocytes were checked for their anti-band-3-antibody loading by means of a protein A-goldtechnique. The in vitro-ageing procedures are introduced as an alternative to the questionable preparation of in vivo aged cells. They include the influences of NaF in various concentrations, of heat (49 °C) and urea. After the treatment with 20 mmol/1 NaF and more, the IgG loading increases significantly. In contrast to that, however, exovesiculation induced by heat and urea lower the IgG loading. Possible mechanisms for the growing accessibility of the epitopes concerned are discussed in connection with changes occurring during the physiological ageing processes.
UR - https://www.scopus.com/pages/publications/0025230551
UR - https://www.scopus.com/inward/citedby.url?scp=0025230551&partnerID=8YFLogxK
U2 - 10.1016/S0065-1281(11)80249-7
DO - 10.1016/S0065-1281(11)80249-7
M3 - Article
C2 - 2113343
AN - SCOPUS:0025230551
SN - 0065-1281
VL - 88
SP - 71
EP - 76
JO - Acta Histochemica
JF - Acta Histochemica
IS - 1
ER -