Synthesis of a novel α-galactosyl ceramide haptenated-lipid antigen, a useful tool in demonstrating the involvement of i NKT cells in the production of antilipid antibodies

Natacha Veerapen, Elizabeth A. Leadbetter, Michael B. Brenner, Liam R. Cox, Gurdyal S. Besra

Producción científica: Articlerevisión exhaustiva

10 Citas (Scopus)

Resumen

A new haptenated derivative of α-galactosyl ceramide (α-GalCer) has been synthesized to assist in the study of the mechanism of T cell help for the production of B cell antibodies. Our synthetic route provides access to an amine intermediate which can easily be extended to generate an array of compounds, useful in various ongoing studies. Herein, we also describe the biological evaluation of the nitrophenyl (NP) haptenated α-GalCer and demonstrate its importance in such mechanistic studies. For instance, in vitro studies showed that NP-α-GalCer stimulates both T and B cell proliferation while in vivo studies in immunized mice showed the production of IgG anti-NP antibodies after exposure to NP-α-GalCer. The interpretation of these results helps toward a better understanding of T cell help for the production of antibodies.

Idioma originalEnglish (US)
Páginas (desde-hasta)741-747
Número de páginas7
PublicaciónBioconjugate Chemistry
Volumen21
N.º4
DOI
EstadoPublished - abr 21 2010
Publicado de forma externa

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Huella

Profundice en los temas de investigación de 'Synthesis of a novel α-galactosyl ceramide haptenated-lipid antigen, a useful tool in demonstrating the involvement of i NKT cells in the production of antilipid antibodies'. En conjunto forman una huella única.

Citar esto