Synergism between photochemical and ionizing radiation effects in MCF-7 cells in vitro

Gulshan Ara, Terence S. Herman, Archana Varshney, Timothy Korbut, Beverly A. Teicher

Resultado de la investigación: Conference contribution


The complexes of platinum tetrachlorodianion with two molecules of rhodamine-123 (Rh-123) or Nile Blue (PtCl4 (Rh-123)2 or PtCl4(Nile Blue)2), have been reported to act as hypoxic cell radiosensitizers of malignant tumors in vitro and in vivo. Both PtCl4(Rh-123)2 and PtCl4(Nile Blue), interact positively in trimodality therapy including each drug, x-ray ana light (400-800 nm) in killing human mammary carcinoma cells (MCF-7) in vitro. The combination treatment results in more than additive killing as assessed by product of the surviving fractions and a significant reduction of the shoulder of the x-ray survival curve. Both of the drugs participate in photodynamic therapy (PDT) in drug and light dose dependent manner. With 15 /μM PtCl4 (Rh-123 )2 plus 24 J/cm2 the survival fraction was 0.03, and with 5 /iM PtCl4(Nile Blue)2 plus 24 J/cm2 the surviving fraction was 0.002 as compared with a surviving fraction of 0.9 ∼ 0.95 with light exposure alone. After treatment with 15 /xM PtCl4(Rh-123 )2 or 5 /xM PtCl4(Nile Blue)2 alone the surviving fractions were ∼ 0.4 or - 0.3 respectively. The primary subcellular targets for the neutral platinum complexes were indicated to be the nuclear DNA as opposed to the mitochondria for Rh-123 or the lysosomes for Nile Blue. Because the nuclear DNA is also the primary target for ionizing radiation, drug plus light plus x-ray might cause supraadditive killing. Both PtCl4(Rh-123)2 and PtCl4(Nile Blue)2 have been reported to be relatively non-toxic in vivo compared to the anionic or cationic compounds currently being used in photodynamic therapy. Based on these results PtCl4(Rh-123 )2 and PtCl4(Nile Blue) 2 have the potential for use in photodynamic therapy and in trimodality therapy.

Idioma originalEnglish (US)
Título de la publicación alojadaLaser-Tissue Interaction III
EditoresSteven L. Jacques
Número de páginas12
ISBN (versión digital)9780819407924
EstadoPublished - ago 7 1992
Publicado de forma externa
EventoLaser-Tissue Interaction III 1992 - Los Angeles, United States
Duración: ene 19 1992ene 24 1992

Serie de la publicación

NombreProceedings of SPIE - The International Society for Optical Engineering
ISSN (versión impresa)0277-786X
ISSN (versión digital)1996-756X


ConferenceLaser-Tissue Interaction III 1992
País/TerritorioUnited States
CiudadLos Angeles

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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