Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

Yuting Li; Francisca M. Acosta; Yumeng Quan; Zhen Li; Sumin Gu; Jean X. Jiang

doi:10.1016/j.xpro.2021.101060

Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

Yuting Li, Francisca M. Acosta, Yumeng Quan, Zhen Li, Sumin Gu, Jean X. Jiang

Department of Biochemistry & Structural Biology

Producción científica: Article › revisión exhaustiva

1 Cita (Scopus)

Resumen

Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).

Idioma original	English (US)
Número de artículo	101060
Publicación	STAR Protocols
Volumen	3
N.º	1
DOI	https://doi.org/10.1016/j.xpro.2021.101060
Estado	Published - mar 18 2022

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Medicine

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title = "Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model",

abstract = "Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).",

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AU - Acosta, Francisca M.

AU - Quan, Yumeng

AU - Li, Zhen

AU - Gu, Sumin

AU - Jiang, Jean X.

PY - 2022/3/18

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Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

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