Resumen
Mutation of surface residues to charged amino acids increases resistance to aggregation and can enable reversible unfolding. We have developed a protocol using the Rosetta computational design package that "supercharges" proteins while considering the energetic implications of each mutation. Using a homology model, a single-chain variable fragment antibody was designed that has a markedly enhanced resistance to thermal inactivation and displays an unanticipated ≈30-fold improvement in affinity. Such supercharged antibodies should prove useful for assays in resource-limited settings and for developing reagents with improved shelf lives.
Idioma original | English (US) |
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Páginas (desde-hasta) | 449-455 |
Número de páginas | 7 |
Publicación | Chemistry and Biology |
Volumen | 19 |
N.º | 4 |
DOI | |
Estado | Published - abr 20 2012 |
Publicado de forma externa | Sí |
ASJC Scopus subject areas
- Drug Discovery
- Molecular Medicine
- Molecular Biology
- Biochemistry
- Clinical Biochemistry
- Pharmacology