TY - JOUR
T1 - Specific and sensitive radioimmunoassay for 3-methoxy-4- hydroxyphenylethyleneglycol(MOPEG)
AU - Kent Keeton, T.
AU - Krutzsch, Henry
AU - Lovenberg, Walter
PY - 1981
Y1 - 1981
N2 - Antibodies that specifically bind the norepinephrine metabolite 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG) were produced in rabbits after injection of a derivative of MOPEG conjugated with bovine thyroglobulin. A sensitive radioimmunoassay was devised with this antiserum, in which as little as 0.5 nanogram of MOPEG can be accurately measured with a final antibody dilution of 1:180. The antibody appears to be specific for MOPEG, since tritiated MOPEG was not displaced from the antibodies by norepinephrine, epinephrine, dopamine, serotonin, or their major metabolites, including MOPEG-sulfate (333 nanograms each).
AB - Antibodies that specifically bind the norepinephrine metabolite 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG) were produced in rabbits after injection of a derivative of MOPEG conjugated with bovine thyroglobulin. A sensitive radioimmunoassay was devised with this antiserum, in which as little as 0.5 nanogram of MOPEG can be accurately measured with a final antibody dilution of 1:180. The antibody appears to be specific for MOPEG, since tritiated MOPEG was not displaced from the antibodies by norepinephrine, epinephrine, dopamine, serotonin, or their major metabolites, including MOPEG-sulfate (333 nanograms each).
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U2 - 10.1126/science.7455697
DO - 10.1126/science.7455697
M3 - Article
C2 - 7455697
AN - SCOPUS:0019350687
VL - 211
SP - 586
EP - 588
JO - Science
JF - Science
SN - 0036-8075
IS - 4482
ER -