SIRT3 deacetylates ATP synthase F1 complex proteins in response to nutrient-and exercise-induced stress

Athanassios Vassilopoulos, J. Daniel Pennington, Thorkell Andresson, David M. Rees, Allen D. Bosley, Ian M. Fearnley, Amy Ham, Charles Robb Flynn, Salisha Hill, Kristie Lindsey Rose, Hyun Seok Kim, Chu Xia Deng, John E. Walker, David Gius

Producción científica: Articlerevisión exhaustiva

140 Citas (Scopus)


Aims: Adenosine triphosphate (ATP) synthase uses chemiosmotic energy across the inner mitochondrial membrane to convert adenosine diphosphate and orthophosphate into ATP, whereas genetic deletion of Sirt3 decreases mitochondrial ATP levels. Here, we investigate the mechanistic connection between SIRT3 and energy homeostasis. Results: By using both in vitro and in vivo experiments, we demonstrate that ATP synthase F1 proteins alpha, beta, gamma, and Oligomycin sensitivity-conferring protein (OSCP) contain SIRT3-specific reversible acetyl-lysines that are evolutionarily conserved and bind to SIRT3. OSCP was further investigated and lysine 139 is a nutrient-sensitive SIRT3-dependent deacetylation target. Site directed mutants demonstrate that OSCPK139 directs, at least in part, mitochondrial ATP production and mice lacking Sirt3 exhibit decreased ATP muscle levels, increased ATP synthase protein acetylation, and an exercise-induced stress-deficient phenotype. Innovation: This work connects the aging and nutrient response, via SIRT3 direction of the mitochondrial acetylome, to the regulation of mitochondrial energy homeostasis under nutrient-stress conditions by deacetylating ATP synthase proteins. Conclusion: Our data suggest that acetylome signaling contributes to mitochondrial energy homeostasis by SIRT3-mediated deacetylation of ATP synthase proteins.

Idioma originalEnglish (US)
Páginas (desde-hasta)551-564
Número de páginas14
PublicaciónAntioxidants and Redox Signaling
EstadoPublished - ago 1 2014
Publicado de forma externa

ASJC Scopus subject areas

  • Molecular Biology
  • Physiology
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology


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