@inbook{85cb79418736482aa43f1d83a41c11cf,
title = "Single-cell library preparation of iPSC-derived neural stem cells",
abstract = "Single-cell RNA-seq technology allows for the identification of heterogeneous cell populations, measures stochastic gene expressions, and identifies highly variable genes. Thus, with this technology it is possible to identify relevant pathways involved in development or in disease progression. Herein, we describe a protocol to capture and process single-cell transcriptomes that will be used for RNA sequencing. This chapter discusses the use of the Fluidigm C1 System and Integrated Fluidic Circuit microfluidics system, TapeStation 4200, SMART-Seq v4, Nextera XT Library Preparation Kit, and AMPure XP beads.",
keywords = "C1, Fluidigm, Library preparation, Neural stem cells, Next-generation sequencing, Nextera XT, RNA-seq, SMART-Seq, Single cell, Single-cell RNA-seq",
author = "Jeffrey Kim and Daadi, {Marcel M.}",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
doi = "10.1007/978-1-4939-9007-8_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "129--143",
booktitle = "Methods in Molecular Biology",
address = "United States",
}