Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

Ande Bao, William T. Phillips, Beth Goins, Howard S. McGuff, Xiangpeng Zheng, F. Ross Woolley, Mohan Natarajan, Cristina Santoyo, Frank R. Miller, Randal A. Otto

Producción científica: Articlerevisión exhaustiva

28 Citas (Scopus)

Resumen

Objective: To develop and characterize a new head and neck cancer animal model. Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too. Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors. Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

Idioma originalEnglish (US)
Páginas (desde-hasta)853-857
Número de páginas5
PublicaciónOtolaryngology - Head and Neck Surgery
Volumen135
N.º6
DOI
EstadoPublished - dic 2006

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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