TY - JOUR
T1 - Serological identification of antigens associated with renal cell carcinoma.
AU - Koroleva, Ekaterina P.
AU - Lagarkova, Maria A.
AU - Mesheryakov, Andrey A.
AU - Scanlan, Matthew J.
AU - Old, Lloyd J.
AU - Nedospasov, Sergei A.
AU - Kuprash, Dmitry V.
PY - 2002/10
Y1 - 2002/10
N2 - Serological analysis of cDNA expression libraries (SEREX) is a technique designed for the identification of tumor specific antigens serologically recognized by the patient immune system. Application of SEREX to different types of tumor led to the identification of a wide range of tumor-associated antigens. Here we used SEREX to search for new antigens associated with renal cancer. The cDNA expression libraries were prepared from two renal cell carcinoma specimens and screened with the autologous patients sera. A total of 96 positive clones representing 66 different genes were isolated, including 18 genes with unknown function. All positive clones were further characterized by reactivity against a panel of sera from healthy donors and patients with renal cancer. As a result, we identified four antigens with a cancer-related serological profile, which may be used for the diagnostics of renal cancer.
AB - Serological analysis of cDNA expression libraries (SEREX) is a technique designed for the identification of tumor specific antigens serologically recognized by the patient immune system. Application of SEREX to different types of tumor led to the identification of a wide range of tumor-associated antigens. Here we used SEREX to search for new antigens associated with renal cancer. The cDNA expression libraries were prepared from two renal cell carcinoma specimens and screened with the autologous patients sera. A total of 96 positive clones representing 66 different genes were isolated, including 18 genes with unknown function. All positive clones were further characterized by reactivity against a panel of sera from healthy donors and patients with renal cancer. As a result, we identified four antigens with a cancer-related serological profile, which may be used for the diagnostics of renal cancer.
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M3 - Article
C2 - 12674932
AN - SCOPUS:0036824352
SN - 1028-7221
VL - 7
SP - 229
EP - 238
JO - Russian journal of immunology : RJI : official journal of Russian Society of Immunology
JF - Russian journal of immunology : RJI : official journal of Russian Society of Immunology
IS - 3
ER -