Sequence variation in the Trichuris trichiura β-tubulin locus: Implications for the development of benzimidazole resistance

A. B. Bennett, T. J.C. Anderson, G. C. Barker, E. Michael, D. A.P. Bundy

    Resultado de la investigación: Articlerevisión exhaustiva

    16 Citas (Scopus)

    Resumen

    Benzimidazole resistance has evolved in a variety of organisms and typically results from mutations in the β-tubulin locus at specific amino acid sites. Despite widespread treatment of human intestinal nematodes with benzimidazole drugs, there have been no unambiguous reports of resistance. However, since β-tubulin mutations conferring resistance are generally recessive, frequencies of resistance alleles less than 30% would be difficult to detect on the basis of drug treatment failures. Here we investigate sequence variation in a 1,079 bp segment of the β-tubulin locus in the human whipworm Trichuris trichiura from 72 individual nematodes from seven countries. We did not observe any alleles with amino acid mutations indicative of resistance, and of 40 point mutations there were only four non-synonymous mutations all of which were singletons. Estimated effective population sizes are an order of magnitude lower than those from another nematode species in which benzimidazole resistance has developed (Haemonchus contortus). Both the lower diversity and reduced population sizes suggest that benzimidazole resistance is likely to evolve less rapidly in Trichuris than in trichostrongyle parasites of livestock. We observed moderate levels of population subdivision (ΦST = 0.26) comparable with that previously observed in Ascaris lumbricoides, and identical alleles were frequently found in parasites from different continents, suggestive of recent admixture. A particularly interesting feature of the data is the high nucleotide diversities observed in nematodes from the Caribbean. This genetic complexity may be a direct result of extensive admixture and complex history of human populations in this region of the world. These data should encourage (but not make complacent) those involved in large-scale benzimidazole treatment of human intestinal nematodes.

    Idioma originalEnglish (US)
    Páginas (desde-hasta)1519-1528
    Número de páginas10
    PublicaciónInternational Journal for Parasitology
    Volumen32
    N.º12
    DOI
    EstadoPublished - nov. 2002

    ASJC Scopus subject areas

    • Parasitology
    • Infectious Diseases

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