TY - JOUR
T1 - Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva
AU - Lam, Y. W.Francis
AU - Chan, Daniel C.N.
AU - Rodriguez, Sandra Y.
AU - Lintakoon, John H.
AU - Lam, Thu Hong
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993/1/29
Y1 - 1993/1/29
N2 - A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 μl) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 μg/ml (r2 ≥ 0.997). Intra-day and inter-day coefficients of variation were <5.5 and ≤9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.
AB - A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 μl) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 μg/ml (r2 ≥ 0.997). Intra-day and inter-day coefficients of variation were <5.5 and ≤9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.
UR - http://www.scopus.com/inward/record.url?scp=0027400179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027400179&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(93)80381-D
DO - 10.1016/0378-4347(93)80381-D
M3 - Article
C2 - 8454696
AN - SCOPUS:0027400179
SN - 0378-4347
VL - 612
SP - 166
EP - 171
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -