RhoB-dependent modulation of postendocytic traffic in polarized madin-darby canine kidney cells

Christine Rondanino, Raul Rojas, Wily G. Ruiz, Exing Wang, Rebecca P. Hughey, Kenneth W. Dunn, Gerard Apodaca

Producción científica: Articlerevisión exhaustiva

28 Citas (Scopus)

Resumen

The Rho family of GTPases is implicated in the control of endocytic and biosynthetic traffic of many cell types; however, the cellular distribution of RhoB remains controversial and its function is not well understood. Using confocal microscopy, we found that endogenous RhoB and green fluorescent protein-tagged wild-type RhoB were localized to early endosomes, and to a much lesser extent to recycling endosomes, late endosomes or Golgi complex of fixed or live polarized Madin-Darby canine kidney cells. Consistent with RhoB localization to early endosomes, we observed that expression of dominant-negative RhoBN19 or dominant-active RhoBV14 altered postendocytic traffic of ligand-receptor complexes that undergo recycling, degradation or transcytosis. In vitro assays established that RhoB modulated the basolateral-to-apical transcytotic pathway by regulating cargo exit from basolateral early endosomes. Our results indicate that RhoB is localized, in part, to early endosomes where it regulates receptor egress through the early endocytic system.

Idioma originalEnglish (US)
Páginas (desde-hasta)932-949
Número de páginas18
PublicaciónTraffic
Volumen8
N.º7
DOI
EstadoPublished - jul 2007
Publicado de forma externa

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Structural Biology
  • Biochemistry
  • Cell Biology

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