Remodeling of tissue-engineered bone structures in vivo

Sandra Hofmann, Monika Hilbe, Robert J. Fajardo, Henri Hagenmüller, Katja Nuss, Margarete Arras, Ralph Müller, Brigitte Von Rechenberg, David L. Kaplan, Hans P. Merkle, Lorenz Meinel

Resultado de la investigación: Articlerevisión exhaustiva

44 Citas (Scopus)


Implant design for bone regeneration is expected to be optimized when implant structures resemble the anatomical situation of the defect site. We tested the validity of this hypothesis by exploring the feasibility of generating different in vitro engineered bone-like structures originating from porous silk fibroin scaffolds decorated with RGD sequences (SF-RGD), seeded with human mesenchymal stem cells (hMSC). Scaffolds with small (106-212 μm), medium (212-300 μm), and large pore diameter ranges (300-425 μm) were seeded with hMSC and subsequently differentiated in vitro into bone-like tissue resembling initial scaffold geometries and featuring bone-like structures. Eight weeks after implantation into calvarial defects in mice, the in vitro engineered bone-like tissues had remodeled into bone featuring different proportions of woven/lamellar bone bridging the defects. Regardless of pore diameter, all implants integrated well, vascularization was advanced, and bone marrow ingrowth had started. Ultimately, in this defect model, the geometry of the in vitro generated tissue-engineered bone structure, trabecular- or plate-like, had no significant impact on the healing of the defect, owing to an efficient remodeling of its structure after implantation.

Idioma originalEnglish (US)
Páginas (desde-hasta)119-129
Número de páginas11
PublicaciónEuropean Journal of Pharmaceutics and Biopharmaceutics
EstadoPublished - sept 2013

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science


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