Release of osteoclast activating factor by normal human peripheral blood leukocytes

Clarence L. Trummel, Gregory R. Mundy, Lawrence G. Raisz

Resultado de la investigación: Articlerevisión exhaustiva

37 Citas (Scopus)


A reliable method for obtaining osteoclast activating factor (OAF) in the culture medium of phytohemagglutinin-activated peripheral blood leukocytes is described. OAF was detected by its ability to stimulate resorption of fetal rat bone in organ culture. Most bone resorbing activity was released by activated leukocytes during the first 24 hours of culture, well before 3H-thymidine incorporation was increased. Cultures maintained for longer than 3 days showed a decrease in OAF activity. Stimulated leukocytes cultured in medium with as little as 0.01 per cent plasma showed increased 3H-thymidine incorporation and released as much OAF as leukocytes cultured with 20 per cent plasma. OAF release was stimulated by pokeweed mitogen and concanavalin A as well as by phytohemagglutinin.

Idioma originalEnglish (US)
Páginas (desde-hasta)1001-1007
Número de páginas7
PublicaciónThe Journal of Laboratory and Clinical Medicine
EstadoPublished - jun. 1975
Publicado de forma externa

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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