Regulatory T cells and TLR9 activation shape antibody formation to a secreted transgene product in AAV muscle gene transfer

Roland W. Herzog, M. Cooper, George Q. Perrin, Moanaro Biswas, Ashley T. Martino, Laurence Morel, Cox Terhorst, Brad E. Hoffman

Producción científica: Articlerevisión exhaustiva

32 Citas (Scopus)

Resumen

Adeno-associated viral (AAV) gene delivery to skeletal muscle is being explored for systemic delivery of therapeutic proteins. To better understand the signals that govern antibody formation against secreted transgene products in this approach, we administered an intramuscular dose of AAV1 vector expressing human coagulation factor IX (hFIX), which does not cause antibody formation against hFIX in C57BL/6 mice. Interestingly, co-administration of a TLR9 agonist (CpG-deoxyoligonucleotide, ODN) but not of lipopolysaccharide, caused a transient anti-hFIX response. ODN activated monocyte-derived dendritic cells and enhanced T follicular helper cell responses. While depletion of regulatory T cells (Tregs) also caused an antibody response, TLR9 activation combined with Treg depletion instead resulted in prolonged CD8+ T cell infiltration of transduced muscle. Thus, Tregs modulate the response to the TLR9 agonist. Further, Treg re-population eventually resolved humoral and cellular immune responses. Therefore, specific modes of TLR9 activation and Tregs orchestrate antibody formation in muscle gene transfer.

Idioma originalEnglish (US)
Número de artículo103682
PublicaciónCellular Immunology
Volumen342
DOI
EstadoPublished - ago 2019
Publicado de forma externa

ASJC Scopus subject areas

  • Immunology

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