Reference transcriptome for deriving marmoset induced pluripotent stem cells

Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav Roy-Choudhury, Jeffrey Kim, Marcel M. Daadi

Producción científica: Chapter

1 Cita (Scopus)

Resumen

Limited access to primary tissue from various nonhuman primate (NHP) species represents a significant unmet need that hampers progress in understanding unique cellular diversity and gene regulation of specific tissues and organs in stem cell translational research. Most comparative biology studies have been limited to using postmortem tissue usually frozen specimens with limited utility for research. The generation of induced pluripotent stem cell (iPSC) lines from somatic cells, such as adult skin or blood cells, offers an alternative to invasive and ethically controversial interventions for acquiring tissue. Pluripotent iPSCs have virtually an unlimited capacity to proliferate and differentiate into all cell types of the body. We are generating high-quality validated NHP iPSC lines to offer to scientific community and facilitate their research programs. We use the non-integrative episomal vector system to generate iPSCs from NHP skin biopsies. In this chapter we describe the validation of NHP iPSC lines by confirming pluripotency and their propensity to differentiate into all three germ layers ectoderm, mesoderm, and endoderm according to established standards and measurable limits for a set of marker genes incorporated into a scorecard.

Idioma originalEnglish (US)
Título de la publicación alojadaMethods in Molecular Biology
EditorialHumana Press
Páginas175-186
Número de páginas12
DOI
EstadoPublished - 2019

Serie de la publicación

NombreMethods in Molecular Biology
Volumen1919
ISSN (versión impresa)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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