Redundant and unique roles of two enhancer elements in the TCRγ locus in gene regulation and γδ T cell development

Na Xiong, Chulho Kang, David H. Raulet

Producción científica: Articlerevisión exhaustiva

54 Citas (Scopus)

Resumen

Many mammalian genes, including those encoding antigen receptors, contain more than one enhancer element. Deleting one element often does not prevent expression, but functional redundancy has never been directly demonstrated by gene targeting of multiple elements. We demonstrate that simultaneous deletion of two enhancer/LCR-like elements in the TCR Cγ1 cluster, HsA and 3′ECγ1, severely diminishes TCRγ transcription, selectively impairs development of γδ thymocyte subsets, but only modestly reduces TCRγ gene rearrangement, while deletion of each element separately has little effect. In contrast to these results in thymocytes, deletion of HsA alone reduces transcription of one Vγ gene specifically in peripheral γδ T cells. Thus, the two elements exhibit functional redundancy in thymocytes but also have unique functions in other settings.

Idioma originalEnglish (US)
Páginas (desde-hasta)453-463
Número de páginas11
PublicaciónImmunity
Volumen16
N.º3
DOI
EstadoPublished - 2002
Publicado de forma externa

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Immunology

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