Redefining thymus medulla specialization for central tolerance

Emilie J. Cosway, Beth Lucas, Kieran D. James, Sonia M. Parnell, Manuela Carvalho-Gaspar, Andrea J. White, Alexei V. Tumanov, William E. Jenkinson, Graham Anderson

Producción científica: Articlerevisión exhaustiva

61 Citas (Scopus)

Resumen

During αβT cell development, the thymus medulla represents an essential microenvironment for T cell tolerance. This functional specialization is attributed to its typical organized topology consisting of a branching structure that contains medullary thymic epithelial cell (mTEC) networks to support negative selection and Foxp3+ T-regulatory cell (T-reg) development. Here, by performing TEC-specific deletion of the thymus medulla regulator lymphotoxin β receptor (LTβR), we show that thymic tolerance mechanisms operate independently of LTβR-mediated mTEC development and organization. Consistent with this, mTECs continue to express Fezf2 and Aire, regulators of intrathymic self-antigens, and support T-reg development despite loss of LTβR-mediated medulla organogenesis. Moreover, we demonstrate that LTβR controls thymic tolerance by regulating the frequency and makeup of intrathymic dendritic cells (DCs) required for effective thymocyte negative selection. In all, our study demonstrates that thymus medulla specialization for thymic tolerance segregates from medulla organogenesis and instead involves LTβR-mediated regulation of the thymic DC pool.

Idioma originalEnglish (US)
Páginas (desde-hasta)3183-3195
Número de páginas13
PublicaciónJournal of Experimental Medicine
Volumen214
N.º11
DOI
EstadoPublished - nov 1 2017

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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