@inbook{37aa5c11302e4315890d05fa1b99889d,
title = "Reconstituting the 4-Strand DNA Strand Exchange",
abstract = "Proteins of the Rad51 family play a key role in homologous recombination by carrying out DNA strand exchange. Here, we present the methodology and the protocols for the 4-strand exchange between gapped circular DNA and homologous linear duplex DNA promoted by human Rad51 and Escherichia coli RecA orthologs. This reaction includes formation of joint molecules and their extension by branch migration in a polar manner. The presented methodology may be used for reconstitution of the medial-to-late stages of homologous recombination in vitro as well as for investigation of the mechanisms of branch migration by helicase-like proteins, e.g., Rad54, BLM, or RecQ1.",
keywords = "Branch migration, DNA double-strand break repair, DNA strand exchange, Holliday junctions, Homologous recombination, Joint molecules",
author = "Mazina, {Olga M.} and Mazin, {Alexander V.}",
note = "Funding Information: We thank Kritika Hanamshet and Nadish Goyal for the feedback on this protocol. We acknowledge funding from the National Cancer Institute of the National Institutes of Health (NIH) Grant numbers CA188347, P30CA056036 (to A.V.M.) and from Drexel Coulter Program (to A.V.M.) for supporting this work. The authors declare no conflicts of interests. Mazina and Mazin, 2004 O.M. Mazina A.V. Mazin Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
doi = "10.1016/bs.mie.2017.11.013",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "285--305",
booktitle = "Methods in Enzymology",
address = "United States",
}