@inbook{fd9aea6ebd064ba7a77cb2fcc3ae5487,
title = "Real-Time Measurement of Energy Metabolism Over Circadian Time Using Indirect Calorimetry-Enabled Metabolic Cages",
abstract = "Indirect calorimetry probes the relationship between fuel consumed and energy produced, and in doing so provides an estimation of whole-body energy expenditure and fuel preference. When assayed continuously in real-time, rhythms appear and illuminate the temporal regulation of energy metabolism by the circadian clock. Here we describe a method for recording circadian energy metabolism in mice using indirect calorimetry-enabled metabolic cages, encompassing mouse entrainment, experimental design, data acquisition and analysis, troubleshooting of common problems, and important considerations. This method is adaptable to the end user{\textquoteright}s equipment and serves as an effective tool to study, for example, mutant mice, dietary interventions, drug treatments, or circadian disruption.",
keywords = "Circadian clock, Circadian rhythm, Energy metabolism, In vivo recording, Indirect calorimetry, Metabolic cage, Real-time recording",
author = "Koronowski, {Kevin B.} and Paolo Sassone-Corsi",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2249-0_20",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "301--310",
booktitle = "Methods in Molecular Biology",
address = "United States",
}