Quantitative proteomic analysis of chromatin-associated factors

Yuzuru Shiio, Robert N. Eisenman, Eugene C. Yi, Sam Donohoe, David R. Goodlett, Ruedi Aebersold

Producción científica: Articlerevisión exhaustiva

69 Citas (Scopus)


A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.

Idioma originalEnglish (US)
Páginas (desde-hasta)696-703
Número de páginas8
PublicaciónJournal of the American Society for Mass Spectrometry
EstadoPublished - jul 2003
Publicado de forma externa

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy


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