Quantitative image-based phosphorus-31 MR spectroscopy for evaluating age-based differences in skeletal muscle metabolites

Shenweng Deng, Erika M. Ripley, Juan A. Vasquez, Devjit Tripathy, Ralph A Defronzo, Geoffrey D Clarke

Producción científica: Conference contribution

2 Citas (Scopus)

Resumen

Purpose: To develop and validate a novel in-vivo phosphorus-31 magnetic resonance spectroscopy (31P-MRS) method to determine the absolute concentrations of phosphocreatine [PCr], inorganic phosphate [Pi], and adenosine triphosphate [ATP] in the vastus lateralis muscle. Materials and Methods: An external 6 mL plastic vial with 850 mM of methylenediphosphonic acid (MDP), fixed to the center of a commercial dual-tuned transmit/receive surface coil, was used to calibrate metabolite concentrations from spectral areas. A 15cm diameter, 4 L cylindrical phantom (35 mM H3PO4) was scanned on a custom coil holder using the same parameters. Reproducibility of the 31P-MRS measurements was determined in volunteers (2M/3F, age = 39.2±21.9 years) while accuracy was determined using phantoms of known concentrations. Eight young subjects (24.5+4.2 years; 4M/4F) and eight older subjects (59.6+4.5 years; 4M/4F) were scanned. Student's t-test was used to compare older versus younger subjects. Results: The percent error between the calculated and known molarity of phantoms was 3.3±1.9%. The mean coefficient of variation for the measurements of [PCr] was 5.2±3.7 %. Phosphorus metabolite concentrations, including [PCr] (25.2±3.4 mM vs. 28.5±3.4 mM, p < 0.005), [ATP] (6.68±0.84 mM vs. 7.71±0.61 mM, p<0.05) and [Pi] (3.18±0.46 mM vs. 2.56±0.55 mM, p<0.05) were significantly lower in older versus younger subjects. A significant, negative correlation was found between [PCr] and BMI (r = -0.50, p < 0.05). Conclusion: Quantitative 31P-MRS measurements reveal previously unappreciated differences in skeletal muscle phosphorus metabolite concentrations between young and older subjects and may provide unique insights when combined with other metabolic tests.

Idioma originalEnglish (US)
Título de la publicación alojadaMedical Imaging 2018
Subtítulo de la publicación alojadaPhysics of Medical Imaging
EditoresTaly Gilat Schmidt, Guang-Hong Chen, Joseph Y. Lo
EditorialSPIE
ISBN (versión digital)9781510616356
DOI
EstadoPublished - 2018
EventoMedical Imaging 2018: Physics of Medical Imaging - Houston, United States
Duración: feb 12 2018feb 15 2018

Serie de la publicación

NombreProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volumen10573
ISSN (versión impresa)1605-7422

Other

OtherMedical Imaging 2018: Physics of Medical Imaging
País/TerritorioUnited States
CiudadHouston
Período2/12/182/15/18

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials

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