TY - JOUR
T1 - Purification of measles virus glycoproteins and their integration into artificial lipid membranes
AU - Casali, P.
AU - Patrick Sissons, J. G.
AU - Fujinami, R. S.
AU - Oldstone, M. B.A.
PY - 1981/1/1
Y1 - 1981/1/1
N2 - We report a simple method for the isolation of the measles virus glycoproteins, and their subsequent incorporation into artifical lipid bilayers. The two viral glycoproteins, HA and F, were isolated in preparative amounts from disrupted purified virus lentil lectin affinity chromatography. The proteins were reconstituted into single bilayer lipid vesicles by: (i) exchanging the non-dialysable detergent Nonidet P40 (NP40) for a dialysable one, octylglucoside, while the proteins were immobilized on the lectin column and (ii) co-dialysis of the eluted glycoproteins in octylglucoside with phosphatidylcholine. The resultant 'virosomes' had visible 'spikes' and possessed haemagglutinating activity. These measles virosomes should provide a useful reagent for studying immune responses to measles virus, independent of the immunosuppressive effects of the whole virus.
AB - We report a simple method for the isolation of the measles virus glycoproteins, and their subsequent incorporation into artifical lipid bilayers. The two viral glycoproteins, HA and F, were isolated in preparative amounts from disrupted purified virus lentil lectin affinity chromatography. The proteins were reconstituted into single bilayer lipid vesicles by: (i) exchanging the non-dialysable detergent Nonidet P40 (NP40) for a dialysable one, octylglucoside, while the proteins were immobilized on the lectin column and (ii) co-dialysis of the eluted glycoproteins in octylglucoside with phosphatidylcholine. The resultant 'virosomes' had visible 'spikes' and possessed haemagglutinating activity. These measles virosomes should provide a useful reagent for studying immune responses to measles virus, independent of the immunosuppressive effects of the whole virus.
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U2 - 10.1099/0022-1317-54-1-161
DO - 10.1099/0022-1317-54-1-161
M3 - Article
C2 - 7288404
AN - SCOPUS:0019830868
SN - 0022-1317
VL - 54
SP - 161
EP - 171
JO - Journal of General Virology
JF - Journal of General Virology
IS - 1
ER -