Protocol for assessing phagocytosis activity in cultured primary murine microglia

Elsie Layman, Jennifer Michelle Parrott, Hye Young Lee

Producción científica: Articlerevisión exhaustiva

Resumen

In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe treatment with lipopolysaccharide (LPS) to induce phagocytosis of fluorescent beads, followed by quantitative analysis using fluorescent imaging and Fiji – ImageJ software. For complete details on the use and execution of this protocol, please refer to Parrott et al.1

Idioma originalEnglish (US)
Número de artículo101881
PublicaciónSTAR Protocols
Volumen3
N.º4
DOI
EstadoPublished - dic 16 2022

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience

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