Proteomic analysis of the left ventricle post-myocardial infarction to identify in vivo candidate matrix metalloproteinase substrates

Andriy Yabluchanskiy, Yaojun Li, Lisandra E. De Castro Brás, Kevin Hakala, Susan T. Weintraub, Merry L. Lindsey

Producción científica: Chapter

5 Citas (Scopus)

Resumen

Left ventricular remodeling post-myocardial infarction (MI) involves a multitude of mechanisms that regulate the repair response. Matrix metalloproteinases (MMPs) are a major family of proteolytic enzymes that coordinate extracellular matrix turnover. MMP-7 or MMP-9 deletion attenuate adverse remodeling post-MI, but the mechanisms have not been fully clarified. Both MMP-7 and MMP-9 have a large number of known in vitro substrates, but in vivo substrates for these two MMPs in the myocardial infarction setting are incompletely identified. Advances in proteomic techniques have enabled comprehensive profiling of protein expression in cells and tissue. In this chapter, we describe a protocol for the proteomic analysis of in vivo candidate MMP substrates in the post-MI left ventricle using two-dimensional electrophoresis, liquid chromatography coupled with tandem mass spectrometry, and immunoblotting.

Idioma originalEnglish (US)
Título de la publicación alojadaCell-Cell Interactions
Subtítulo de la publicación alojadaMethods and Protocols
EditorialHumana Press
Páginas185-199
Número de páginas15
ISBN (versión impresa)9781627036030
DOI
EstadoPublished - 2013

Serie de la publicación

NombreMethods in Molecular Biology
Volumen1066
ISSN (versión impresa)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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