Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNAfMet binding to 40S ribosomes

N. K. Gupta; B. Chatterjee; A. Majumdar

doi:10.1016/S0006-291X(75)80215-4

Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNA_f^Met binding to 40S ribosomes

N. K. Gupta
, B. Chatterjee
, A. Majumdar

Producción científica: Article › revisión exhaustiva

11 Citas (Scopus)

Resumen

A Millipore filtration assay method has been developed for studies of codon directed Met-tRNA_f^Met binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNA_f^Met binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.

Idioma original	English (US)
Páginas (desde-hasta)	797-805
Número de páginas	9
Publicación	Biochemical and Biophysical Research Communications
Volumen	65
N.º	2
DOI	https://doi.org/10.1016/S0006-291X(75)80215-4
Estado	Published - jul 22 1975
Publicado de forma externa	Sí

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

Acceder al documento

10.1016/S0006-291X(75)80215-4

Otros archivos y enlaces

Citar esto

@article{8beb290f685641779e92785627e36fb8,

title = "Protein synthesis in rabbit reticulocytes XII: Requirement of mRNA (AUG codon) for Met-tRNAfMet binding to 40S ribosomes",

abstract = "A Millipore filtration assay method has been developed for studies of codon directed Met-tRNAfMet binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNAfMet binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.",

author = "Gupta, \{N. K.\} and B. Chatterjee and A. Majumdar",

note = "Funding Information: This work was supported by NIH Research Grants (GM-18796, GM-22079) and an American Cancer Society Grant NP-I13. The authors thank Mr. Y. C. Chen for generous supplies of DNA-template and RNA polymerase.",

year = "1975",

month = jul,

day = "22",

doi = "10.1016/S0006-291X(75)80215-4",

language = "English (US)",

volume = "65",

pages = "797--805",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Protein synthesis in rabbit reticulocytes XII

T2 - Requirement of mRNA (AUG codon) for Met-tRNAfMet binding to 40S ribosomes

AU - Gupta, N. K.

AU - Chatterjee, B.

AU - Majumdar, A.

N1 - Funding Information: This work was supported by NIH Research Grants (GM-18796, GM-22079) and an American Cancer Society Grant NP-I13. The authors thank Mr. Y. C. Chen for generous supplies of DNA-template and RNA polymerase.

PY - 1975/7/22

Y1 - 1975/7/22

N2 - A Millipore filtration assay method has been developed for studies of codon directed Met-tRNAfMet binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNAfMet binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.

AB - A Millipore filtration assay method has been developed for studies of codon directed Met-tRNAfMet binding to 40S ribosomes. Under the assay conditions, the stimulation of Met-tRNAfMet binding to Millipore filters by added 40S ribosomes is strongly dependent on the presence of AUG codon, and partially purified peptide chain initiation factors. Crude 0.5 M KC1 ribosomal wash is inactive in catalyzing this binding reaction due to the presence of an inhibitor in this preparation.

UR - https://www.scopus.com/pages/publications/0016732239

UR - https://www.scopus.com/pages/publications/0016732239#tab=citedBy

U2 - 10.1016/S0006-291X(75)80215-4

DO - 10.1016/S0006-291X(75)80215-4

M3 - Article

C2 - 1148015

AN - SCOPUS:0016732239

SN - 0006-291X

VL - 65

SP - 797

EP - 805

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -