Prevalence of the GABA(A) receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration

Ashok K. Mehta; Maharaj K. Ticku

doi:10.1016/S0169-328X(99)00020-0

Prevalence of the GABA(A) receptor assemblies containing α₁-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration

Ashok K. Mehta, Maharaj K. Ticku

Department of Pharmacology

Resultado de la investigación: Article › revisión exhaustiva

25 Citas (Scopus)

Resumen

The anti-α₁ antibody elicited higher immunoprecipitation (%) values of the [³H]flunitrazepam and [³H]muscimol binding activity in the rat cerebellum vs. cerebral cortex, whereas immunoprecipitation values for [³H]Ro 15-4513 and [³H]zolpidem were comparable in these brain regions. Chronic ethanol administration neither changed the radioligand binding to the immunoprecipitated pellet nor the percentage immunoprecipitation values, thereby indicating that chronic ethanol did not result in down-regulation of the GABA(A) receptor assemblies containing α₁-subunit.

Idioma original	English (US)
Páginas (desde-hasta)	194-199
Número de páginas	6
Publicación	Molecular Brain Research
Volumen	67
N.º	1
DOI	https://doi.org/10.1016/S0169-328X(99)00020-0
Estado	Published - abr 6 1999

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/S0169-328X(99)00020-0

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Prevalence of the GABA(A) receptor assemblies containing α₁-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation : Lack of modulation by chronic ethanol administration. / Mehta, Ashok K.; Ticku, Maharaj K.

En: Molecular Brain Research, Vol. 67, N.º 1, 06.04.1999, p. 194-199.

Resultado de la investigación: Article › revisión exhaustiva

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abstract = "The anti-α1 antibody elicited higher immunoprecipitation (%) values of the [3H]flunitrazepam and [3H]muscimol binding activity in the rat cerebellum vs. cerebral cortex, whereas immunoprecipitation values for [3H]Ro 15-4513 and [3H]zolpidem were comparable in these brain regions. Chronic ethanol administration neither changed the radioligand binding to the immunoprecipitated pellet nor the percentage immunoprecipitation values, thereby indicating that chronic ethanol did not result in down-regulation of the GABA(A) receptor assemblies containing α1-subunit.",

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author = "Mehta, {Ashok K.} and Ticku, {Maharaj K.}",

note = "Funding Information: We thank Ms. Elena Wright for valuable technical help, and Ms. Sadie Phillips for excellent secretarial help. This work was supported by funds from National Institute on Alcohol and Alcohol Abuse Grant AA 04090.",

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