Preparation of protein gel matrix and angiogenesis of vascular endothelial cell in the gel

Vitronectin was purified from human plasma by heparin affinity chromatography and rough plasma protein containing fibrinogen was isolated from human plasma by (NH₄)₂SO₄ precipitation. Protein gel was generated by addition of thrombin to the complex composed of plasma protein, bovine fetal serum and DMEM. Bovine aortic endothelial cell (BAEC) was plated on the gel matrix and the growth, vascular formation of endothelial cell in the gel were observed. The results demonstrated that BAEC can adhere to the gel surface and grow normally as grow on the culture plate, while under the induction of bFGF, BAEC can migrate into the gel and form vascular-like structure in the gel matrix. Many vascular-like structures can link to each other to form capilary net structure.