Preparation of normalized cDNA libraries for 454 titanium transcriptome sequencing

Zhao Lai, Yi Zou, Nolan C. Kane, Jeong Hyeon Choi, Xinguo Wang, Loren H. Rieseberg

Producción científica: Chapter

9 Citas (Scopus)


Transcriptome sequencing from cDNA libraries has been extensively and efficiently used to analyze sequence variation in protein-coding genes (Expressed Sequence Tags) in eukaryote species. Rapid advances in next-generation sequencing (NGS) technology, in terms of cost, speed, and throughput, allow us to address previously unanswerable questions in the fields of ecology, evolution, and systematics using these genomic tools. Transcriptome sequencing from individuals across different populations and species enables researchers to study the evolution of gene sequence variation at a population genomics level. In this chapter, we describe a customized protocol that has been successfully optimized for the development of normalized cDNA libraries in eukaryote systems suitable for Roche 454 GS FLX sequencing, requiring only small quantities of starting material.

Idioma originalEnglish (US)
Título de la publicación alojadaData Production and Analysis in Population Genomics
Subtítulo de la publicación alojadaMethods and Protocols
EditorialHumana Press
Número de páginas15
ISBN (versión impresa)9781617798696
EstadoPublished - 2012
Publicado de forma externa

Serie de la publicación

NombreMethods in Molecular Biology
ISSN (versión impresa)1064-3745

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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