TY - JOUR
T1 - Pregnancy-driven cardiovascular maternal miR-29 plasticity in obesity
AU - Schlabritz-Loutsevitch, N.
AU - Apostolakis-Kyrus, K.
AU - Krutilina, R.
AU - Hubbard, G.
AU - Kocak, M.
AU - Janjetovic, Z.
AU - Sathanandam, S.
AU - Slominski, A. T.
AU - Mari, G.
AU - Dick, E.
N1 - Publisher Copyright:
© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Background: Obesity in pregnancy (MO) is a risk factor for maternal and/or fetal cardiovascular system disorders. This study evaluated maternal CVS expression of microRNA-29 family and its target molecules in MO to test the hypotheses: CVS miR-29 concentrations are increased in pregnancy and decreased in MO. Methods: Non-pregnant (n=4), pregnant obese (POb, n=4), and pregnant non-obese (PnOb, n=4) baboons (Papio spp.) were studied. Maternal left ventricle (LV), left atrium (LA), and aortic arch (AA) were collected at the end of gestation. Expression of MiR-29 and elastin (ELN) mRNA were quantified. Results: LA miR-29 (a, c) expression was highest in PnOb. In the LV, miR-29b expression trended lower (P=.059) for PnOb animals. ELN mRNA expression correlated positively with miR-29b expression in AA (r=.76, P=.03). Conclusion: Maternal obesity diminishes miR-29 adaptation to pregnancy. Pharmacologic, tissue-specific targeting of miRNA-29 may represent a strategy for prevention and treatment of MO complications.
AB - Background: Obesity in pregnancy (MO) is a risk factor for maternal and/or fetal cardiovascular system disorders. This study evaluated maternal CVS expression of microRNA-29 family and its target molecules in MO to test the hypotheses: CVS miR-29 concentrations are increased in pregnancy and decreased in MO. Methods: Non-pregnant (n=4), pregnant obese (POb, n=4), and pregnant non-obese (PnOb, n=4) baboons (Papio spp.) were studied. Maternal left ventricle (LV), left atrium (LA), and aortic arch (AA) were collected at the end of gestation. Expression of MiR-29 and elastin (ELN) mRNA were quantified. Results: LA miR-29 (a, c) expression was highest in PnOb. In the LV, miR-29b expression trended lower (P=.059) for PnOb animals. ELN mRNA expression correlated positively with miR-29b expression in AA (r=.76, P=.03). Conclusion: Maternal obesity diminishes miR-29 adaptation to pregnancy. Pharmacologic, tissue-specific targeting of miRNA-29 may represent a strategy for prevention and treatment of MO complications.
KW - maternal obesity
KW - non-human primate
KW - vascular remodeling
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U2 - 10.1111/jmp.12236
DO - 10.1111/jmp.12236
M3 - Article
C2 - 27627870
AN - SCOPUS:84987735816
SN - 0047-2565
VL - 45
SP - 297
EP - 303
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
IS - 6
ER -